Coronavirus refers to one strand positive RNA virus that's ubiquitous in nature. There are six sorts of coronaviruses that have already been proved to attack humans, 229E, OC43, NL63 and HIU1, and a couple of more pathogenic coronavirus strains discovered in recent years, i.e. the acute respiratory syndrome coronavirus in 2002-2003, SARS CoV, Middle East respiratory syndrome coronavirus (MERS COV) in 2012. The novel coronavirus (SARS-CoV-2) outbroke in 2019 is that the seventh. Novel coronavirus pneumonia (COVID-19) may be a novel coronavirus-caused lung inflammation, to which humans are generally susceptible.
Respiratory droplets and get in touch with are the most routes of transmission. The infected patients usually show pneumonia-like symptoms (fever, cough, dyspnea, etc.), diarrhea, and other gastrointestinal symptoms, followed by severe acute respiratory tract infection. Some cases will have acute respiratory distress with serious respiratory complications, even resulting in death.
Since the outbreak COVID-19, till Washington's Birthday, 2020, the statistics of the planet Health Organization (WHO) showed that 77,794 patients were confirmed to be infected, of which 76,392 cases were confirmed in China. So as to accurately diagnose and record the spread of this virus, doctors need rapid and reliable in vivo & in vitro diagnostic methods.
Overview of the virus laboratory detection technology
All substances, except prions, contain nucleic acids that include desoxyribonucleic acid (DNA) and RNA (RNA). The SARS-CoV-2 only contains RNA, whose specific RNA sequence may be a marker for distinguishing it from other pathogens.
The most commonly applied method by the Coronavirus Testing Center to detect the precise sequence of novel coronavirus is the fluorescence quantitative polymerase chain reaction (PCR). Consistent with the etiological diagnosis, either the results of clinical diagnosis with RT-PCR defection of respiratory specimens or liquid samples show positive, or the sequencing result reveals high similarity thereto of SARS-CoV-2, maybe a confirmed case.
Immunoassay technologies
Immunocolloid technology is usually applied in local medical units and for on-site test for its quick operation and practicability characters, which may assist with the diagnosis of SARS-CoV-2 and other epidemics。
Chemiluminescence receives popularity during tests of clinical samples thanks to its characteristics of high affinity, wide linear range, and fast speed.
The enzyme-linked immunosorbent assay (ELISA) is typically wont to detect the precise anti-virus antibodies in patients at progressive and convalescent stages.
Operations requirements of COVID-19 Test Lab conducting immunoassay are relatively lower, making it more suitable for the screening and epidemiologic investigation. For the false-negative results, combined tests on SARS-CoV-2 specific antibodies (IgG/IgM) can explain whether the patient has been infected, which further assists with the diagnosis of the suspected cases with negative PCR results. Creative Biolabs is capable of developing first-rate IVD antibodies targeting this novel coronavirus, which may apply to varied immunoassays like ELISA. Many CROs are now receiving an outsized volume of orders from researchers performing on the study of SARS-CoV-2 diagnostic methods, so on define this highly communicable disease.
SARS-CoV-2, as a replacement virus that seriously endangers public health and safety after Ebola, Zika, avian influenza, and swine flu virus, becomes the main target of worldwide attention during a short time. Successful virus isolation has opened a door to developing accurate diagnostic methods and effective vaccines. As there's no specific treatment method, rapid and accurate detection methods for early diagnosis and control are essential. Biology, thanks to its high specificity and powerful sensitivity, provides precise and fast identification methods alongside epidemiologic information. Meanwhile, pathogenic microphysical and immunological detection technologies should join to realize a rapid and clear diagnosis.
For more info :- COVID-19 Testing Center